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Dr.Shereen Mohamed Fouad Elkholy

PhD in Molecular Biology/ Microbiology from University of Ulm Germany Assistant Professor Department of Biology (MOI:English) College of Science and Humanity Studies in Alkharj
  • Kharj
  • 011-588-8941
  • s.elkholy@psau.edu.sa
  • Curriculum Vitae
  • Publications

Education

  • B.Sc., Faculty of Science, Ain Shams University, Egypt, Microbiology, 1995 M.Sc.: Faculty of Science, Ain Shams University, Egypt, Molecular Biology. 1999 Ph.D.: Institute of Medical Microbiology and Immunology, University of Ulm, Germany, Human Genetics, 2003.

  • B.Sc., Faculty of Science, Ain Shams University, Egypt, Microbiology, 1995 M.Sc.: Faculty of Science, Ain Shams University, Egypt, Molecular Biology. 1999 Ph.D.: Institute of Medical Microbiology and Immunology, University of Ulm, Germany, Human Genetics, 2003.

Research Interests

  • I'm interest in the following research topics:
  • 1. Production of plant-derived and edible vaccines
  • 2. Identification of the most suitable reference genes for gene expression normalization.
  • 3. Using Immunoinformatics to Design a mRNA Vaccine against the Spike Glycoprotein of SARS-CoV-2
  • 4. Using Bioinformatics to investigate the novel SARS-CoV-2 variants and their impacts on infectivity

Experience

  • 2021 - until now - Head of Research (Professor) - Agricultural Genetic Engineering Research Institute
  • 2010 - 2021 - Senior Researcher - Agricultural Genetic Engineering Research Institute
  • 2010 - until now - Assistant Professor - Prince Sattam bin abdulaziz University
  • 2003 - 2010 - Researcher - Agricultural Genetic Engineering Research Institute
  • 2005 - 2006 - Postdoctoral Research Associate - University of Masssachusetts Medical School , MA, USA
  • 2004 - 2005 - Postdoctoral Research Associate - Worcester Polytechnic Institute, MA, USA
  • 2002 - 2003 - Postdoctoral Research Associate - University of Regensburg, Germany
  • 1999 - 2002 - Ph.D. Candidate - University of Ulm, Germany
  • 1997 - 1999 - Research Assistant - Agricultural Genetic Engineering Research Institute

Responsibilities

  • Secretary of the Biology Department Board (2014-present) Supervisor of the Research Service Unit at Deanship of Scientific Research (2018-2020)

Publications

  • 1- Shereen F. Elkholy (2021). Using Bioinformatics to investigate the novel SARS-CoV-2 variants and their impacts on infectivity. Egyptian Journal of Genetics and Cytology. 50(2):171-192
  • 2- Shereen F. Elkholy (2021). Using Immunoinformatics to Design a mRNA Vaccine against the Spike Glycoprotein of SARS-CoV-2. Romanian Biotechnological Letters. 26(5): 2901-2915.
  • 3- Shereen F. Elkholy (2021). Comparative Computational Analysis of SARS-CoV-2 Nucleocapsid Epitope with Taxonomically Related Corona Viruses. Romanian Biotechnological Letters. 26(4): 2737-2750.
  • 4- Nahed A. A. Ibrahim, Asmaa H. Hassan, Omaima A. Sharaf, Mohamed E. Saad, Shereen F. Elkholy and Ghada A. Abu El-Heba (2021). A novel Medicago truncatule AKT1-null mutant impairs Potassium & Sodium Ions Uptake and Affects Root Nodulation. Egyptian Journal of Botany. 61(1) 303-318.
  • 5- Shereen F. Elkholy. (2020). Identification of the most suitable reference genes for gene expression normalization in safflower (Carthamus tinctorius L.) treated with gibberellin. Research Journal of Biotechnology. 15(5) 24-32.
  • 6- Shereen F. Elkholy. (2019). Using Bioinformatics to Study Homology Between Herpes Simplex Virus Type 1 Glycoprotein C Gene and Herpes Simplex Virus Type 2 Glycoprotein F Gene. American Journal of Biomedical Science and Research. 2(2) 326-335.
  • 7- Pravej Alam., Mahmoud A. Sharaf-Eldin, Shereen F. Elkholy, Parvaiz Ahmad and Nasser S. Alkhalifah. (2019). Establishment of cell suspension culture system and production of bioactive compounds in Moringa oleifera. Z Arznei- Gewurzpfla.23(1) 28-32.
  • 8- Mahmoud A. Sharaf-Eldin, Pravej Alam1, Shereen F. Elkholy, Abdul Samad, Aftab Alam and Manuel C. Palada (2019). Impact of Different Drying Methods on Nutritional Value, Flavonoid Content and Antioxidant Activity of Moringa oleifera. Z Arznei- Gewurzpfla.23(1) 22-27.
  • 9- Mahmoud A. Sharaf-Eldin, Pravej Alam and Shereen F. Elkholy (2019) Molecular and chemical characterization of mutant and nonmutant genotypes of saffron grown in Saudi Arabia. Food Science and Nutrition. 7(1) 247-255.
  • 10- Shereen F. Elkholy and Zainab M. Almutairi (2018). Effect of Priming with Gibberellin on Germination and expression of GA Biosynthesis gene of tomato (Solanum lycopersicum L.) seedlings under salt Stress. International Journal of Biotechnology and Research. 8(3): 11-22.
  • 11- Pravej Alam, Mahmoud A. Sharaf-Eldin, Shereen F. Elkholy and Parvaiz Ahmad (2018). Physiochemical impact of a magnetic field on A. annua L. alters its genomic profile and enhances artemisinin content. Pak. J. Bot., 50(3): 1023-1028.
  • 12- Alam, P., Zainul Abdeen Khan, Malik Zainul Abdin, Jawaid A. Khan, Parvaiz Ahmad, Shereen F. Elkholy, Mahmoud A. Sharaf-Eldin. (2017). Efficient regeneration and improved sonicated assisted Agrobacterium transformation (SAAT) method for Catharanthus roseus. 3 Biotech 7(26): 1-10.
  • 13- Pravej Alam, Malik Zainul Abdin, Naveed Ahmad, Mirza Sarwar Baig, Mahmoud A. Sharaf-Eldin and Shereen F. Elkholy. (2017). Functional characterization of cytochrome P450 variant (CYP71) isolated from Artemisia annua L. Plants. Int. J. Pharm. Res. Allied Sci. 6(1):22-46.
  • 14- Alam, P., Elkholy, S. F., Hosokawa, M., Mahfouz, S. A., & Sharaf-Eldin, M. A. (2016). Simultaneous extraction and rapid HPLC based quantification of crocin and safranal in saffron (Crocus sativusL.). International Journal of Pharmacy and Pharmaceutical Sciences, 8(10), 224-227.
  • 15- Alam, P., Elkholy, S. F., Mahfouz, S. A.,Alam, P.,& Sharaf-Eldin, M. A. (2016). HPLC based estimation and extraction of rutin, quercetin and gallic acid in Moringaoleifera plants grown in Saudi Arabia. Journal of Chemical and Pharmaceutical Research, 8(8):,1243-1246.
  • 16- Alam, P., Sharaf-Eldin, M. A., Elkholy, S. F., & Abdin, M. Z. (2016). The effect of over-expression of rate limiting enzymes on the yield of artemisinin in Artemisia annua. Rendiconti Lincei, 27(2), 311-319.
  • 17- M.A. Sharaf-Eldin, A. Al-Tamimi , P. Alam , S.F. Elkholy and J.R. Jordan (2015) Genetic relatedness of artichoke (Cynara scolymus L.) hybrids using random amplified polymorphic DNA (RAPD) fingerprinting. Genetics and Molecular Research, 28(4): 18431-18439.
  • 18- Pravej Alam, Kamaluddin Mahmoud, A. Sharaf-Eldin, Shereen F. Elkholy, Malik Zainul Abdin (2015) The effect of over-expression of rate limiting enzymes on the yield of artemisinin in Artemisia annua. Rend. Fis. Acc. Lincei, 27(2): 311-319. 19- Mahmoud Sharaf-Eldin, Shereen elkholy, Munteka Hosokawa, Kai Yanagawa, Eiji Nawata, Keiichi Takagi, Jose- Antonio Fernandez. (2015) Saffron Flowers with Augmented Number of Stigmata. Journal of medicine and spice plants, 20(2): 84-87.
  • 20- Shereen Elkholy, Mahmoud Sharaf-Eldin and Yi Wang (2010).Correlation of the Expression Level of Amorpha Diene Synthase Gene (ADS) with the Artemisinin Level in Artemisia annua L. Plant. Egyptian Journal of Genetics and Cytology, 39: 1-13.
  • 21- Shereen Elkholy and Mahmoud Sharaf-Eldin (2009). Comparison of the Expression Level of Two Sesquiterpene Cyclases Genes in the Transformed Hairy Roots of Artemisia annua L. Plant. Egyptian Journal of Genetics and Cytology, 33:333-348.
  • 22- Sharaf-Eldin, M.A., Elkholy, S., Fernández, J.-A., Junge, H., Cheetham, R.D., Guardiola, J.L. and Weathers, P.J. (2008) Bacillus subtilis FZB24® affects flower quantity and quality of saffron (Crocus sativus L.), Planta Medica, 74 (10) pp.1316-1320.
  • 23- Shereen F. Elkholy, Roba M. Ismail, Ahmed Bahieldin, Atef S. Sadik and Magdy A. Madkour (2008). Expression of Hepatitis B Surface Antigen (HBsAg) gene in Transgenic Banana (Musa sp.), Arab Journal of Biotechnology, 12:291-302.
  • 24- Pamela J. Weathers, Shereen Elkholy and Kristin K. Wobbe. (2006). Artemisinin: The biosynthetic pathway and its regulation in Artemisia annua, a terpenoid-rich species. Invitro Cellular and Developmental Biology; 42: 309-317.
  • 25- Shereen Elkholy, Shireen K. Assem, Hanaiya A. El-Itriby and Magdy A. Madkour. (2004). Expression of Hepatitis B surface antigen (HBsAg) in transgenic maize (Zea mays L.). Egyptian Journal of Genetics and Cytology, 33(2):333-348.
  • 26- Reinhold Schirmbeck, Marcin Kwissa, Nicolas Fissolo, Shereen Elkholy, Petra Riedl Jörg Reimann. (2002) Priming polyvalent immunity by DNA vaccines expressing chimeric antigens with a stress proteins-capturing viral J domain. The FASEB Journal; 16(9): 1108-10.
  • 27- Reinhold Schirmbeck, Detlef Stober, Shereen Elkholy, Petra Riedl Jörg Reimann. (2002) The immunodominant, Ld-restricted T cell response to hepatitis B surface antigen (HBsAg) efficiently suppresses CD8+ T cell responses to different Dd-, Kd- and Kb-restricted HBsAg epitopes. The Journal of Immunology; 168(12): 6253-62.
  • 28- Petra Riedl*, Shereen Elkholy*, Jörg Reimann and Reinhold Schirmbeck. (2002) Priming biologically active antibody responses against an isolated, conformational viral epitope by DNA vaccination. The Journal of Immunology; 169(3): 1251-60. *S.E and P.R contributed equally to this work
  • 29- Shereen Elkholy, Petra Riedl, Marcin Kwissa, Jörg Reimann and Reinhold Schirmbeck (2002). Selective expression of immunogenic, VLP-derived epitopes binding antibodies. Intervirology; 45(4-6): 251-9.

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