المرفقات
المحاضرات
الإسم تفاصيل أخرى
Introduction to Microbiology

Definition and scope of Microbiology - history and recent developments-the origin of microbial life - theory of spontaneous generation- Pasteur’s Tyndall experiments- fermentation studies- contributions of Leeuwenhoek, Lister, Robert Koch, Winogeadsky, Beijerinck, Christian Hansen - impact and scope of microbiology.

Microscopy

Definitions- resolving power- focal length- resolution-simple- compound light microscopy - dark ground - phase contrast - fluorescence and electron microscopy

Anatomy of prokaryotic cell

Structure and functions of cell wall- cytoplasmic membrane- cilia- flagella- capsule- cytoplasmic inclusions- plasmids and ribosomes- Bacterial endospores and method of sporulation.

Anatomy of Eukaryotic cell

Structure and functions of external cell coverings- cytoplasmic matrix- microfilaments-nucleus, nucleous –ribosomes-mitochonderia -chloroplasts filaments-microtubules-endoplasmic reticulum -golgi apparatus-lysosomes -endocytosis-differences between prokaryotic and eukaryotic cell.

Bacterial Taxonomy and classification

Microbial kingdoms - Five kingdom concepts - cell theory - Binomial nomenclature of microbes - species concept - Description of organisms - Classical approach with examples. Classical techniques of microbial identification - morphological, physiological and biochemical properties.

Bacterial cultivation

Culture media- types- Pure culture technique- Streak plate- pour plate- spread plate- decimal dilution- pure and mixed cultures-storage of bacterial stocks-Quality control.

Microbial growth

Batch and continuous culture - factors affecting growth - Growth determination - Growth curve- binary fission- Measurement of cell number, measurement of cell mass. Factors affecting growth. 

Microbial Metabolism-I

Basic concepts of metabolism - mechanism of ATP formation - substrate phosphorylation. Electron transport, phosphorylation, photophosphorylation. energy production during oxidation of organic substrate - amino acid and assimilatory pathway. 

Microbial Metabolism-II

Bacterial enzymes - coenzymes - isoenzymes - structure, classification and properties. 

Antimicrobial Chemotherapy

Antibiotics definition- source - classification – different mode of action - Antimicrobial resistance - tests for sensitivity to antimicrobial agents and its quality control

Sterilization and Disinfection

Sterilization – principles- methods of sterilization - dry heat, moist heat, filtration, Tyndallization, Pasteurization, Radiation- Disinfection- types of disinfectants.

Introduction to Virology

Structure and morphology of viruses- properties-classification-replication-viral pathogenesis.

Introduction to Parasites

Classification -General characteristics of protozoa-Medically important protozoa-General characteristics of medically important helminthes. 

Introduction to Fungi

General properties of fungi- Classification (morphological and etiological) and clinical- Structure and morphology of fungi- fungal diseases.

Introduction to Computers in Medical Field

History of Computers, Basic Anatomy of Computers. Computers in Taxonomy and Systemic Data Analysis in Microbiology. Computers in clinical microbiology - Computer applications in fermentation - Technology Computers in Drug - Designing using various softwares in Drug designing.

الخطة الدراسية
MLAB 352  -  General Microbiology
عدد الوحدات عدد الساعات
نظري عملي
15 75 60
وصف المقرر

Course Specifications

Institution: Salman Bin Abdulaziz University                               

                                                            

College/Department : College of Applied Medical Sciences/ Department of  Medical Lab Sciences

A. Course Identification and General Information

1.  Course title and code:  General Microbiology (MLAB 352)

2.  Credit hours:  4 (3 1 0)

3.  Program(s) in which the course is offered. (If general elective available in many programs indicate this rather than list programs): Medical Laboratory Sciences

                                                                                                               

4.  Name of faculty member responsible for the course: Dr. Mohamudha Parveen Rahamathulla

                                                                                       

5.  Level/year at which this course is offered: 5th level  / 1st semester 1434/35H

6.  Pre-requisites for this course (if any): MLAB 245

7.  Co-requisites for this course (if any): None

8.  Location if not on main campus: Wadi Al Dawaser 

9.  Mode of Instruction (mark all that apply)  ✓

80

 


     a. Traditional classroom                                         What percentage? 

20

 


     b. Blended (traditional and online)                        What percentage?

--

--

 


     c.  e-learning                                                           What percentage?

-

 


-

     d. Correspondence                                                  What percentage?

--

--

     f.   Other                                                                  What percentage?

Comments:  Nil

      


B  Objectives

1.  What is the main purpose for this course?

The main purpose of this course is to provide students of applied medical sciences with a comprehensive and up-to-date guide to General Microbiology including basic scientific knowledge as well as cognitive, psychomotor and interpersonal and numerical skills in the most reliable, easy, attractive and illustrated manner.

         At the end of this course the students should be able to

1-      Understand the principle of light, dark ground, phase contrast, fluorescent and electron,

Microscopy and be able to set up a light microscope with dark ground and phase contrast,

2-      Explain how microorganisms are classified into major taxonomic categories.

3-      List the structural differences between eukaryotic and prokaryotic cells.

4-      Identify the five common shapes of bacterial cells as: coccus, bacillus, cocco bacillus, spirillum, and pleomorphic.

5-      Identify the structure and function of bacterial components: cell wall, capsule, slime layer, fimbriae, pili, flagella, plasmid, chromosome, and ribosome.

6-      Describe and illustrate the difference between Gram positive and Gram negative bacteria.

7-      Understand the structure, physiology, metabolism and genetic of medically important bacteria.

8-      Explain the major metabolic pathways for micro-organisms.

9-      Understand bacterial growth and reproduction.

10-  Describe the environmental conditions of growth.

11-  Explain generation time and diagram the phases of the bacterial growth curve.

12-  Identify and demonstrate how to control microbial growth.

13-  Define sterilization, disinfection, and antisepsis and list examples of each.

14-  Identify and evaluate various physical and chemical control methods.

15-  Understand general properties of viruses including morphology and classification.

16-  Understand general properties of parasitology, including morphology, and classification.

17-  Understand  basic mycology.

18-  Understand the applications of computers in biology.

2.  Briefly describe any plans for developing and improving the course that are being implemented.  (e.g. increased use of IT or web based reference material,  changes in content as a result of new research in the field)

o   Continuous updating of the information, knowledge and skills included in the course through continuous search for the new knowledge and skills available in recent publications (books, researches, internet and others).

o   Verifying the information resources.

o   Continuous improvements in teaching methods as well as encouraging the students to participate effectively in the lectures.

o   Continuous evaluation of the course content, student level and establish plans accordingly.

C.  Course Description (Note:  General description in the form to be used for the Bulletin or handbook should be attached)

This course provides the students with a basic knowledge of morphology, structures, classifications, modes of growth, and growth requirements, disease relationship of various micro-organisms. These include fungi, viruses, parasites, and detailed studies on bacteria. The students will be acquainted with the methods of sterilization, disinfection, antiseptics and antimicrobial agents.

Topics to be Covered

List of Topics

No. of week

Contact Hours

1. Introduction to Microbiology : Definition and scope of Microbiology - history and recent developments-the origin of microbial life - theory of spontaneous generation- Pasteur’s Tyndall experiments- fermentation studies- contributions of Leeuwenhoek, Lister, Robert Koch, Winogeadsky, Beijerinck, Christian Hansen - impact and scope of microbiology.

      1st

3

Lab: Acquainted with the equipment and tools- sources of contamination- cleaning of glass ware.

         

           2

2. Microscopy: Definitions- resolving power- focal length- resolution-simple- compound light microscopy - dark ground - phase contrast - fluorescence and electron microscopy.

     2nd

3

Lab: Microscopy- Handling techniques- care and maintenance of microscope.

         

           2

3. Anatomy of prokaryotic cell: Structure and functions of cell wall- cytoplasmic membrane- cilia- flagella- capsule- cytoplasmic inclusions- plasmids and ribosomes- Bacterial endospores and method of sporulation.

      3rd

3

Lab: Biosafety in microbiology lab- laboratory safety measures- aseptic techniques.

         

           2

4. Anatomy of Eukaryotic cell: Structure and functions of external cell coverings- cytoplasmic matrix- microfilaments-nucleus, nucleous –ribosomes-mitochonderia -chloroplasts filaments-microtubules-endoplasmic reticulum -golgi apparatus-lysosomes -endocytosis-differences between prokaryotic and eukaryotic cell.

    

      4th

3

Lab: Motility demonstration : Hanging drop preparation, wet mount, dark field microscopy, semisolid agar, Craigies tube method.

         

           2

5. Bacterial Taxonomy and classification: Microbial kingdoms - Five kingdom concepts - cell theory - Binomial nomenclature of microbes - species concept - Description of organisms - Classical approach with examples. Classical techniques of microbial identification - morphological, physiological and biochemical properties.

      5th

3

Lab: Culture technique - media preparation-solid and liquid media- sterility check.

         

           2

6. Bacterial cultivation:  Culture media- types- Pure culture technique- Streak plate- pour plate- spread plate- decimal dilution- pure and mixed cultures-storage of bacterial stocks-Quality control.

      6th

3

Lab: Pure culture technique- Steak and pour plate method

         

           2

7. Microbial growth: Batch and continuous culture - factors affecting growth - Growth determination - Growth curve- binary fission- Measurement of cell number, measurement of cell mass. Factors affecting growth.

       7th

3

Lab: Determination of bacterial growth curve

         

           2

8. Microbial Metabolism-I: Basic concepts of metabolism - mechanism of ATP formation - substrate phosphorylation. Electron transport, phosphorylation, photophosphorylation. energy production during oxidation of organic substrate - amino acid and assimilatory pathway.

      8th

3

Lab: Smear preparation- simple staining.

         

           2

9. Microbial Metabolism-II: Bacterial enzymes - coenzymes - isoenzymes - structure, classification and properties. 

       9th

3

Lab: Gram staining and Acid –fast staining.

         

           2

10. Antimicrobial Chemotherapy: Antibiotics definition- source - classification – different mode of action - Antimicrobial resistance - tests for sensitivity to antimicrobial agents and its quality control.

      

       10th

3

Lab: Spore staining and negative staining.

         

           2

11. Sterilization and Disinfection: Sterilization – principles- methods of sterilization - dry heat, moist heat, filtration, Tyndallization, Pasteurization, Radiation- Disinfection- types of disinfectants.

        11th

3

Lab: Antibiotic sensitivity testing: Disc diffusion test - Quality control with standard strains.

         

           2

12. Introduction to Virology: Structure and morphology of viruses- properties-classification-replication-viral pathogenesis.

        12th

3

Lab: Micrometry-Determination of size of Bacteria, yeast. Fungal filaments

         

           2

13. Introduction to Parasites: Classification -General characteristics of protozoa-Medically important protozoa-General characteristics of medically important helminthes.

        13th

3

Lab: Physiological characteristics: IMVic test, MRVP test, H2S, Oxidase, catalase, urease test, gelatin liquefaction.

         

           2

14. Introduction to Fungi: General properties of fungi- Classification (morphological and etiological) and clinical- Structure and morphology of fungi- fungal diseases.

       14th

3

Lab:  Morphology of fungi, yeasts- slide culture techniques.

         

           2

15. Introduction to Computers: History of Computers, Basic Anatomy of Computers. Computers in Taxonomy and Systemic Data Analysis in Microbiology. Computers in clinical microbiology - Computer applications in fermentation - Technology Computers in Drug - Designing using various softwares in Drug designing.

     

       15th

3

Lab: Microsoft, Powerpoint, automation in microbiology

         

           2

Course Learning Outcomes, Assessment Methods, and Teaching Strategy work together and are aligned. They are joined together as one, coherent, unity that collectively articulate a consistent agreement between student learning, assessment, and teaching.

The National Qualification Framework provides five learning domains. Course learning outcomes are required. Normally a course has should not exceed eight learning outcomes which align with one or more of the five learning domains. Some courses have one or more program learning outcomes integrated into the course learning outcomes to demonstrate program learning outcome alignment. The program learning outcome matrix map identifies which program learning outcomes are incorporated into specific courses.  

On the table below are the five NQF Learning Domains, numbered in the left column.

First, insert the suitable and measurable course learning outcomes required in the appropriate learning domains (see suggestions below the table). Second, insert supporting teaching strategies that fit and align with the assessment methods and intended learning outcomes. Third, insert appropriate assessment methods that accurately measure and evaluate the learning outcome. Each course learning outcomes, assessment method, and teaching strategy ought to reasonably fit and flow together as an integrated learning and teaching process. Fourth, if any program learning outcomes are included in the course learning outcomes, place the @ symbol next to it.

Every course is not required to include learning outcomes from each domain.


NQF Learning Domains

 And Course Learning Outcomes

Course Teaching

Strategies

Course Assessment

Methods

1.0

Knowledge

1.1

By the end of the course, students should be able to:

1-      Understand the principle of light, dark ground, phase contrast, fluorescent and electron microscopy and be able to set up a light microscope with dark ground and phase contrast.

2-      Explain how microorganisms are classified into major taxonomic categories.

3-      List the structural differences between eukaryotic and prokaryotic cells.

4-      Identify the five common shapes of  bacterial cells as : coccus, bacillus, coccobacillus,spirillum, and pleomorphic.

5-      Identify the structure and function of bacterial components: cell wall, capsule, slime layer, fimbriae, pili, flagella, plasmid, chromosome, and ribosome.

6-      Describe and illustrate the  difference  between gram positive and gram negative bacteria.

7-      Understand the structure, physiology, metabolism and genetic of medically important bacteria.

8-      Explain the major metabolic pathways for microorganisms.

9-      Understand  bacterial growth and reproduction.

10-  Describe the environmental conditions of growth.

11-  Explain generation time and diagram the phases of the bacterial growth curve.

12-  Identify and demonstrate how to control  microbial growth.

13-  Define sterilization ,disinfection, and antisepsis and list examples of each.

14-  Identify and evaluate various physical and chemical control methods.

15-  Understand general properties of viruses including morphology and classification.

16-  Understand general properties of parasitology, including  morphology, and classification.

17-  Understand  basic mycology and computers

-  Classroom lectures

-  Power point lectures

-  Group discussion 

-  Assignment

-  Laboratory work

-  e-learning

-          Examinations

-          Assignment

-          Presentation

-          Class participation.

2.0

Cognitive Skills

2.1

1-      Ability of the students to search and learn scientific terms

2-      The ability to compare and contrast between important groups of microorganisms

3-      The ability to apply the gained knowledge to design tests involving microbial  investigations to assess and solve microbiological problems in human infections.

4-      The ability to think critically and make reasonable judgments by analyzing, combining and evaluating

-          Lectures

-          Practical and laboratory work

-          Individual assignments

-          Evaluation of model lab reports

-          Written exam

-          Practical exam

-          Assignment marks

3.0

Interpersonal Skills & Responsibility

3.1

1- Acquire the basic skills of microbiological laboratory and standard techniques for microscopic examination, staining, cultivation and identification of organisms.

2- Acquire the laboratory skills for performing antimicrobial susceptibility testing by proper

choice of antibiotics and sensitivity testing methods as well as interpretations of the laboratory results.

3- Recognize and realize the significance of safety in microbiology laboratory and the applications of laboratory safety measures.

4- Communicate effectively with teaching staff, colleagues and the community.

5- Demonstrate appropriate attitude towards teaching staff, colleagues and patients.

6- Identify the importance of team work.

7- Apply information technology.

8-  Time management

9- To be able for joining theoretical and practical

studies of microbiology with the different and application in industry, agriculture and medicine.etc

-          Students lecture

-          Group discussion

-          Practical classes

-          Motivation of the students

-          Written exam

-          Practical exam

-          Assignment marks

4.0

Communication, Information Technology, Numerical

4.1

1-Discussing and presenting lab results or finding in laboratory.

2- Use information and communication technology

3-Use the sources of biomedical information to remain current with the advances in  knowledge & practice.

4-Express themselves freely and adequately by improving their descriptive capabilities and enhancing their communication skills.

5-Apply English language as needed for appropriate learning and communication in relation to medicine.

6- Communicate clearly, sensitively and effectively with the patients and their families or relatives and with colleagues from different health care professions.

-          Teaching and learning in English to improve student communication skills.

-          Application of simple computer program to improve student IT skills.

-          Internet search and assignments.

-          Written exams should be answered in English.

5.0

Psychomotor

5.1

1- Demonstrate and describe safety precautions required in microbiology laboratories to protect workers.

2- Acquire the basic skills of microbiological laboratory standard techniques for diagnosis of microbial infection.

3- Demonstrate proper a septic technique.

4- Demonstrate proper hand washing, disinfectant procedures, and spill cleanup.

5- Select the correct use of laboratory equipments used for working with microorganisms.

6- Identify the correct staining methods and apply these methods used to identify microorganisms.

7- Develop the proficiency in the use of microscope in the laboratory.

8- Prepare different media in the laboratory.

9- Perform and evaluate the use of different biochemical tests in the laboratory.

10-Experiment how microorganisms are collected, inoculated, cultured, incubated and autoclaved.

11- Perform the correct inoculation techniques

-          Develop through study skills for scientific and qualitative methods.

-          Applied throughout the reporting of laboratory exercises.

-          Engage students in analysis and evaluation of their practical work.

-          Practical and professional skills are assessed through coursework task and reports.

-          Assessed laboratories.

-          Laboratories practicals and reports.

-          Laboratory practical exam.

5. Schedule of Assessment Tasks for Students During the Semester

Assessment task (e.g. essay, test, group project, examination, speech, oral presentation, etc.)

Week Due

Proportion of Total Assessment

1

First Exam

7th week

10%

2

Second Exam

11th week

10%

3

Participation

13th week

10%

4

Practical Exam

14th week

20%

  5

Final Theoretical Exam

15th week

50%

D. Student Academic Counseling and Support

1. Arrangements for availability of faculty and teaching staff for individual student consultations and academic advice. (include amount of time teaching staff are expected to be available each week)

4 hours / week for individual student consultations and academic advice.

Wednesday 10.00 am – 12.00 pm

Thursday 10.00 am – 12.00 pm

المتطلبات

Code & No       : MLAB 352

الرقم والرمز    : 352 مختب

Course             : General Microbiology

اسم المقرر    : علم الأحياء الدقيقة العامة

Credits             : 4 (3 1 0)

الساعات       : 4 (3 1 0)

Pre-requisite   : MLAB 245

Introduction to Clinical Lab Sciences

متطلب سابق  : 245 مختب

مقدمة عن علوم المختبرات الإكلينيكية

يشمل هذا المقرر الأساسيات العامة لعلم الأحياء الدقيقة ويدرس تقسيمات الميكروبات المختلفة من فيروسات وبكتيريا وفطريات وطفيليات والشكل التركيبي لكل مجموعة إضافة لدراسة خصائصها، بالإضافة لتدريس الطالب طرق التعقيم والتطهير واستخدام المضادات الحيوية.

This course provides the students with a basic knowledge of morphology, structures, classifications, modes of growth, and growth requirements, disease relationship of various micro-organisms. These include fungi, viruses, parasites, and detailed studies on bacteria. The students will be acquainted with the methods of sterilization, disinfection, antiseptics and antimicrobial agents.

Requirements

Indicate requirements for the course including size of classrooms and laboratories (i.e. number of seats in classrooms and laboratories, extent of computer access etc.)

1.  Accommodation (Classrooms, laboratories, demonstration rooms/labs, etc.)

-          Lecture room

-          Practical lab

-          Data show

-          Smart  Board

2. Computing resources (AV, data show, Smart Board, software, etc.)

Data shows, projectors, color and laser printers, smart board in classrooms.

3. Other resources (specify, e.g. if specific laboratory equipment is required, list requirements or attach list)

·         Bacterial  and fungal culture media

·         Antibiotic disks

·         E-strips

·         Test tubes

·         Zone measuring scale

·         Autoclave

·         Hot air oven

·         Diagnostic kits

·         Lamina flow hood

·         Connection with hospital to obtain the specimens and for training personnel

·         Permanent teaching slides

- Library supplied with reference textbooks, electronic resources.

G   Course Evaluation and Improvement Processes

1 Strategies for Obtaining Student Feedback on Effectiveness of Teaching

- First Exam

- Second Exam

- Student Participation

- Final Exam

- Students' comments during official hours.

- Students feedback obtained at the end of the semester using student course evaluation

2  Other Strategies for Evaluation of Teaching by the Program/Department Instructor

-          Peer evaluation and academic advising by the instructor

3  Processes for Improvement of Teaching

- Improve course by obtaining new information from updated books and internet.

- Continuous updating of course contents according to the previous course report.

 - Training of the faculty.

- Periodical department revision of its method of teaching.

 - Considering evaluation of faculty by student.

 - Encourage student to participate in learning process to become active participant.

- Encourage student to do more seminars and do creative assignments.

4. Processes for Verifying Standards of Student Achievement (e.g. check marking by an independent  member teaching staff of a sample of student work, periodic exchange and remarking of tests or a sample of assignments with staff at another institution)

- By conducting standards exams.

- Taking a sample of assignments and exams to determine validity and reliability

- Corrected a sample from the leaves of the tests.

- Assigning group of member teaching the same course to grade same questions for various students

5. Describe the planning arrangements for periodically reviewing course effectiveness and planning for improvement.

- Collecting all reports and evaluations at the end of the year for a reviewing purpose.

- Conducting a workshop to presents finding of reports and evaluation to share knowledge.

- The test committee in the department revises the scores of the students.

- The head of the department and faculty should take the responsibility of implementing the proposed changes.

- Student feedback obtained at the end of the semester using student course evaluation survey.

- Compare syllabi and courses description with other universities that available on the net.

الكتاب

1.      Pelczar Jr. M.J. Chan. E.C.S and Kreig. N.R (2006). "Microbiology"- 5th Edition Mc Graw Hill Inc. New York.

2.      David, B.D., Delbecco,. R., Eisen, H.N and Ginsburg, H.S (1990) "Microbiology" 5th Edition. Harper & Row, New York. 

المؤلفين

References Materials:

  1. Lansing M. Prescott., John. P. Harley., Donald A, Klein, "Microbiology"-Mc Graw Hill Inc. New York.

  1. Robert F.Boyd., "General Microbiology" 2nd Edition., Times MIRROR/Moshy College Publishing Virginia.

  1. Bailey and Scott’s Diagnostic Microbiology. Baron and Finegold. Latest Edition.

4.      Color Atlas of diagnostic Microbiology. Maza LD, Pezzlo M, Baron E. Mosby-year book Inc., USA. Latest Edition

5.      Manual of Clinical Microbiology. Murray PR, et al. ASM Press. Latest Edition.

  1. Manual for the Laboratory Identification and Antimicrobial Susceptibility Testing of Bacterial Pathogens of Public Health Importance in the Developing World. Perilla MJ, et al. CDC and WHO

  1. Rajagopalan 1987. Understanding Computers Tata McGraw -Hill Publishing Company Ltd, New Delhi.

المحاضرين

Dr.Mohamudha Parveen Rahamathulla

احتساب الدرجات
نوع التقييم الدرجة
   1 .  I term test 10 % ()
   2 .  II term test 10 % ()
   3 .  Participation 10 % ()
   4 .  Practical Exam 20 % ()
   5 .  Final Theory Exam 50 % ()
الأهداف
الموضوعات